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1) Manage, operate, and perform routine and preventive maintenance, calibration and quality control on all facility instrumentation, including multi-parameter flow analyzers, high-speed cell sorters, confocal, and wide-field microscopes
2) Diagnose faults, troubleshoot problems, and coordinate instrument repairs with engineers and service personnel
3) Provide extensive one-on-one and group training to new and existing users on instrument operation, sample preparation, and relevant software
4) Consult with users on experimental design, panel design (for flow cytometry), data acquisition, fluorophore selection, gating strategies and interpretation of results
5) Collaborate with researchers and the facility manager to develop, evaluate, and
optimize new techniques and analytical workflows, such as high-parameter immunophenotyping, spectral flow cytometry, 4-way/single cell sorting and live-cell imaging
6) Contribute to the well-organized functioning of the laboratory and assist in maintaining the stock levels by purchasing consumables and supplies in consultation with other members of the facility
7) Assist with the development and implementation of standard operating procedures
(SOPs) for the facility
8) Provide support and training for data analysis using common software packages
(e.g., FlowJo, FCS Express, ImageJ)
9) Ensure proper data backup, archiving, instrument booking schedules and management
procedures are followed
10) Assist in general lab organization, managing stock levels, ordering consumables
and supplies, and tracking service contracts
11) Ensure compliance with institutional safety policies and good working practices
(such as biosafety levels)
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